Influence of the Synthesis Scheme of Nanocrystalline Cerium Oxide and Its Concentration on The Biological Activity of Cells Providing Wound Regeneration

In the ongoing search for practical uses of rare-earth metal nanoparticles, cerium dioxide nanoparticles (nanoceria) have received special attention. The purpose this research was to study biomedical effects nanocrystalline forms oxide obtained by different synthesis schemes and evaluate effect concentrations nanoceria (from 10−2 10−6 M) on cells involved in regeneration skin cell structures such as fibroblasts, mesenchymal stem cells, keratinocytes. Two methods preparation were investigated: (1) CeO-NPs-1 precipitation from aqueous solutions (III) nitrate hexahydrate citric acid (2) CeO-NPs-2 hydrolysis ammonium hexanitratocerate (IV) under conditions thermal autoclaving. According X-ray diffraction, transmission electron microscopy, dynamic light scattering data, CeO2-1 consists individual particles (3–5 nm) their aggregates with diameters 60–130 nm. CeO2-2 comprises small 8–20 nm diameter, which consist 2–3 size. Cell cultures human keratinocytes cocultured sols (10−2, 10−3, 10−4, 10−5, mol/L). metabolic activity all types investigated MTT test after 48 72 h, whereas proliferative cytotoxicity determined quantitative culture counting live/dead test. A dependence biological method concentration revealed. Data respect optimal sol achieve highest used cultures. Hypotheses about mechanisms structure a fundamentally new medical device accelerated healing wounds formulated. significantly change types—from suppression pronounced stimulation. best through cocultivation citrate (CeO-NPs-1) at 10−3–10−4 M.